Hotspot

ycf3-trnS(GGA)

LSC · 46297-47098 · 802 bp

Back to Schrebera

Pi 0.00579

MarkerSeek score 59.20

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 12
pi: 0.00579
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	890-905 bp	1.0	73.5
ycf3-trnS(GGA)_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	911-926 bp	1.0	73.0
ycf3-trnS(GGA)_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	861-876 bp	1.0	72.8
ycf3-trnS(GGA)_p4	CATTTATCCGCGATCTAGGCA	CTCGGCCATCTCTCCTACAT	47.6	55.0	58.0	58.4	838-853 bp	1.0	71.4
ycf3-trnS(GGA)_p5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	891-906 bp	1.0	71.2