Hotspot

trnS(GCU)-trnG(GCC)

LSC · 9400-10110 · 711 bp

Back to Schrebera

Pi 0.0108

MarkerSeek score 56.70

Primer available yes

Label trnS(GCU)-trnG(GCC)

Feature Metadata

label_name: trnS(GCU)-trnG(GCC)
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 17
pi: 0.0108
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnG(GCC)_p1	CAATCCGACGCTTTAGTCCA	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.0	56.6	798-818 bp	1.0	64.4
trnS(GCU)-trnG(GCC)_p2	AATCCGACGCTTTAGTCCAC	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.0	56.6	797-817 bp	1.0	64.4
trnS(GCU)-trnG(GCC)_p3	ATCAACGGAACCGGAAAGAG	AGACCGAAAGACCCTTTAACT	50.0	42.9	57.9	56.6	862-882 bp	1.0	63.4
trnS(GCU)-trnG(GCC)_p4	CATCAACGGAACCGGAAAGA	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.2	56.6	863-883 bp	1.0	62.6
trnS(GCU)-trnG(GCC)_p5	TCATCAACGGAACCGGAAAG	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.2	56.6	864-884 bp	1.0	62.6