Hotspot

trnD-GUC

LSC · 32938-33011 · 74 bp

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Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnD-GUC

Feature Metadata

label_name: trnD-GUC
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC_p1	TCCCTTTCACAGAAAATGGAGA	TCCCGCCTATGTCAGTTACA	40.9	50.0	57.3	58.1	236 bp	1.0	57.4
trnD-GUC_p2	TCCCTTTCACAGAAAATGGAGA	AGTCCCGCCTATGTCAGTTA	40.9	50.0	57.3	57.8	238 bp	1.0	57.3
trnD-GUC_p3	TCCCTTTCACAGAAAATGGAGA	GTCCCGCCTATGTCAGTTAC	40.9	55.0	57.3	57.8	237 bp	1.0	56.8
trnD-GUC_p4	TCCCTTTCACAGAAAATGGAGA	TATCAGTCCCGCCTATGTCA	40.9	50.0	57.3	57.6	242 bp	1.0	55.6
trnD-GUC_p5	CCCTTTCACAGAAAATGGAGAG	TCCCGCCTATGTCAGTTACA	45.5	50.0	57.0	58.1	235 bp	1.0	54.8