Hotspot

rpoC1

LSC · 22230-25098 · 2869 bp

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Pi 0.00349

MarkerSeek score 57.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2869
variable_sites: 10
pi: 0.00349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GAGAGACCCGAATTCACACC	TCCAGATTCATAGGAAGGAAGC	55.0	45.5	58.0	57.3	2982 bp	1.0	58.6
rpoC1_p2	GGAGAGACCCGAATTCACAC	TCCAGATTCATAGGAAGGAAGC	55.0	45.5	58.0	57.3	2983 bp	1.0	58.6
rpoC1_p3	GAGAGACCCGAATTCACACC	CCAGATTCATAGGAAGGAAGCT	55.0	45.5	58.0	57.3	2981 bp	1.0	58.5
rpoC1_p4	GGAGAGACCCGAATTCACAC	CCAGATTCATAGGAAGGAAGCT	55.0	45.5	58.0	57.3	2982 bp	1.0	58.5
rpoC1_p5	GATCCTAGTTGACCCGGAGA	TCCAGATTCATAGGAAGGAAGC	55.0	45.5	57.9	57.3	2998 bp	1.0	58.1