Hotspot

ycf1-trnN-GUU

IRa · 133755-134098 · 344 bp

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Pi 0

MarkerSeek score 26.50

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 344
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATAGAGTCCGACCACAACGA	ACGACCAGTCAGTTAACAGC	50.0	50.0	58.2	57.8	477 bp	1.0	77.4
ycf1-trnN-GUU_p2	ATAGAGTCCGACCACAACGA	AGGATTCGAACCTACGACCA	50.0	50.0	58.2	58.2	490 bp	1.0	77.4
ycf1-trnN-GUU_p3	ACCACAACGACCGAATTCAT	ACGACCAGTCAGTTAACAGC	45.0	50.0	57.8	57.8	467 bp	1.0	77.4
ycf1-trnN-GUU_p4	ACCACAACGACCGAATTCAT	AGGATTCGAACCTACGACCA	45.0	50.0	57.8	58.2	480 bp	1.0	77.3
ycf1-trnN-GUU_p5	ATAGAGTCCGACCACAACGA	GGATTCGAACCTACGACCAG	50.0	55.0	58.2	57.8	489 bp	1.0	77.0