Hotspot

rpl20-rps12

LSC · 72032-72796 · 765 bp

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Pi 0.00261

MarkerSeek score 60.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 2
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CCCCCGAAAGCTTGATGTAA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	870 bp	1.0	78.2
rpl20-rps12_p2	CCCCCGAAAGCTTGATGTAA	CGAAATCCCCTGCTCTTCAG	50.0	55.0	57.9	58.3	902 bp	1.0	76.5
rpl20-rps12_p3	TCTTGAATGAGTCCCCCGAA	CGCCGAGGAACATGTACTAG	50.0	55.0	58.3	57.9	882 bp	1.0	76.4
rpl20-rps12_p4	CCCCCGAAAGCTTGATGTAA	TGTTACGAAATCCCCTGCTC	50.0	50.0	57.9	57.6	907 bp	1.0	76.0
rpl20-rps12_p5	TTGAATGAGTCCCCCGAAAG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.5	57.9	880 bp	1.0	75.3