Hotspot

petA-psbJ

LSC · 65932-66735 · 804 bp

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Pi 0.00249

MarkerSeek score 60.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 2
pi: 0.00249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAGGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.5	57.6	864-865 bp	1.0	73.5
petA-psbJ_p2	TCCGTTACGTGTACAAGGTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.3	57.6	948-949 bp	1.0	71.6
petA-psbJ_p3	CCGTTACGTGTACAAGGTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.3	57.6	947-948 bp	1.0	71.6
petA-psbJ_p4	GAGAGGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.2	57.6	863-864 bp	1.0	71.2
petA-psbJ_p5	GAGAGGGTTCAATTGTCCGAA	ATTCCGGATTGGGTTCATCC	47.6	50.0	57.9	57.6	863-864 bp	1.0	68.8