Hotspot

trnD-GUC-trnY-GUA

LSC · 33012-33469 · 458 bp

Back to Schotia

Pi 0.00673
MarkerSeek score 50.90
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
458
variable_sites
3
pi
0.00673
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA ATTGACTCTTCCTGGGTCGA 50.0 50.0 58.5 58.1 573-585 bp 1.0 77.8
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA TTGACTCTTCCTGGGTCGAT 50.0 50.0 58.5 58.1 572-584 bp 1.0 77.8
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 516-528 bp 1.0 76.6
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 515-527 bp 1.0 76.6
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GATTGACTCTTCCTGGGTCG 50.0 55.0 58.5 57.7 574-586 bp 1.0 75.8