Hotspot

petN-psbM

LSC · 30585-31866 · 1282 bp

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Pi 0.00156

MarkerSeek score 59.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1282
variable_sites: 2
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1444 bp	1.0	67.7
petN-psbM_p2	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1393 bp	1.0	65.9
petN-psbM_p3	TCCCTCGTAGTATGGGGAAG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1394 bp	1.0	65.9
petN-psbM_p4	GGGGGCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1466 bp	1.0	65.9
petN-psbM_p5	AGGGGGCATAATTCACATGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1467 bp	1.0	65.8