Hotspot

atpH-atpI

LSC · 14523-15921 · 1399 bp

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Pi 0.00501

MarkerSeek score 61.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1399
variable_sites: 7
pi: 0.00501
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1520-1668 bp	1.0	79.2
atpH-atpI_p2	CGCAATCCCTTCTACAGCTT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1574-1722 bp	1.0	79.1
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1486-1634 bp	1.0	79.1
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1487-1635 bp	1.0	77.9
atpH-atpI_p5	CAATAGAAGCAAGCCCGACA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.3	57.9	1521-1669 bp	1.0	77.3