Hotspot

trnS-GCU-trnG-UCC

LSC · 8332-9179 · 848 bp

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Pi 0.00472
MarkerSeek score 61.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
848
variable_sites
4
pi
0.00472
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ATGAATCAAACGAGGGACCC 50.0 50.0 58.0 57.6 961-982 bp 1.0 76.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ATGAATCAAACGAGGGACCC 50.0 50.0 58.0 57.6 960-981 bp 1.0 76.9
trnS-GCU-trnG-UCC_p3 CAGATTAGCAATCCGACGCT ATGAATCAAACGAGGGACCC 50.0 50.0 58.1 57.6 969-990 bp 1.0 75.8
trnS-GCU-trnG-UCC_p4 AGCAATCCGACGCTTTAGTC ATGAATCAAACGAGGGACCC 50.0 50.0 58.4 57.6 963-984 bp 1.0 74.1
trnS-GCU-trnG-UCC_p5 CGACGCTTTAGTCCACTCAG ATGAATCAAACGAGGGACCC 55.0 50.0 58.4 57.6 956-977 bp 1.0 73.9