Hotspot

ycf1

Genome · 104476-109971 · 5496 bp

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Pi 0.0113

MarkerSeek score 46.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5496
variable_sites: 62
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTGGTTGATTAGACCCCAA	CCCAAAACGGACCTTCCTTT	40.0	50.0	54.4	58.3	232 bp	1.0	51.7
ycf1_p2	TTTTGGTTGATTAGACCCCA	CCCAAAACGGACCTTCCTTT	40.0	50.0	54.4	58.3	233 bp	1.0	51.7
ycf1_p3	TTTTGGTTGATTAGACCCCAA	CCCAAAACGGACCTTCCTTT	38.1	50.0	55.3	58.3	233 bp	1.0	50.6
ycf1_p4	TTGGTTGATTAGACCCCAAT	CCCAAAACGGACCTTCCTTT	40.0	50.0	54.2	58.3	231 bp	1.0	49.7
ycf1_p5	TTTGGTTGATTAGACCCCAAT	CCCAAAACGGACCTTCCTTT	38.1	50.0	55.1	58.3	232 bp	1.0	48.8