Hotspot

psbA

Genome · 140-1201 · 1062 bp

Back to Schoepfia

Pi 0.00471

MarkerSeek score 47.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 5
pi: 0.00471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGGACTCGTATACACTAAGACGA	CGACTAGTTTCGGGTTCGAG	43.5	55.0	58.0	58.1	1331 bp	1.0	55.6
psbA_p2	AGGACTCGTATACACTAAGACGA	GTGCTAGAACTTTGGCTCGT	43.5	50.0	58.0	58.2	1744-1759 bp	1.0	55.6
psbA_p3	AGGACTCGTATACACTAAGACGA	TGAGAATTCTCGTGCTTGGG	43.5	50.0	58.0	57.9	1144 bp	1.0	55.5
psbA_p4	AGGACTCGTATACACTAAGACGA	AATTCTCGTGCTTGGGAGTC	43.5	50.0	58.0	57.9	1140 bp	1.0	55.5
psbA_p5	AGGACTCGTATACACTAAGACGA	GAATTCTCGTGCTTGGGAGT	43.5	50.0	58.0	57.9	1141 bp	1.0	55.5