Hotspot

ycf1-trnN-GUU

Genome · 109972-110410 · 439 bp

Back to Schoepfia

Pi 0.0117

MarkerSeek score 64.50

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: Genome
length_bp: 439
variable_sites: 5
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	AAAGTCCCGCCAAATTGACT	ACGACCAGTCAGTTAACAGC	45.0	50.0	58.0	57.8	550-561 bp	1.0	81.1
ycf1-trnN-GUU_p2	AAAGTCCCGCCAAATTGACT	AGGATTCGAACCTACGACCA	45.0	50.0	58.0	58.2	563-574 bp	1.0	81.0
ycf1-trnN-GUU_p3	AAAGTCCCGCCAAATTGACT	GGATTCGAACCTACGACCAG	45.0	55.0	58.0	57.8	562-573 bp	1.0	80.6
ycf1-trnN-GUU_p4	CCCGCCAAATTGACTCTCTT	ACGACCAGTCAGTTAACAGC	50.0	50.0	58.2	57.8	545-556 bp	1.0	79.7
ycf1-trnN-GUU_p5	CCCGCCAAATTGACTCTCTT	AGGATTCGAACCTACGACCA	50.0	50.0	58.2	58.2	558-569 bp	1.0	79.6