Hotspot

rpl14-rpl16

Genome · 75153-75254 · 102 bp

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Pi 0.0294

MarkerSeek score 63.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: Genome
length_bp: 102
variable_sites: 3
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	TGAAATGGGCGGAATAGAGG	50.0	50.0	58.0	57.4	262 bp	1.0	75.6
rpl14-rpl16_p2	TCAATTCTCGAGCCCCACTA	TGAAATGGGCGGAATAGAGG	50.0	50.0	58.1	57.4	257 bp	1.0	74.7
rpl14-rpl16_p3	ACACATCAATTCTCGAGCCC	GAAATGGGCGGAATAGAGGA	50.0	50.0	58.0	57.1	261 bp	1.0	73.2
rpl14-rpl16_p4	TTCTCGAGCCCCACTATTGT	TGAAATGGGCGGAATAGAGG	50.0	50.0	58.4	57.4	253 bp	1.0	72.4
rpl14-rpl16_p5	TCAATTCTCGAGCCCCACTA	GAAATGGGCGGAATAGAGGA	50.0	50.0	58.1	57.1	256 bp	1.0	72.4