Hotspot

psbE-petL

Genome · 59656-60437 · 782 bp

Back to Schoepfia

Pi 0.00653

MarkerSeek score 64.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 782
variable_sites: 5
pi: 0.00653
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGAACGTTCGCCTGTG	AGTCGTATCTTGCTCATACCA	50.0	42.9	58.3	56.3	894-902 bp	1.0	58.0
psbE-petL_p2	CAAAAGAACGTTCGCCTGTG	TCGTATCTTGCTCATACCAACA	50.0	40.9	58.3	57.3	892-900 bp	1.0	58.0
psbE-petL_p3	ATCAGCAAAAGAACGTTCGC	AGTCGTATCTTGCTCATACCA	45.0	42.9	57.7	56.3	899-907 bp	1.0	57.5
psbE-petL_p4	ATCAGCAAAAGAACGTTCGC	TCGTATCTTGCTCATACCAACA	45.0	40.9	57.7	57.3	897-905 bp	1.0	57.5
psbE-petL_p5	CAAAAGAACGTTCGCCTGTG	AGTCGTATCTTGCTCATACCAA	50.0	40.9	58.3	57.0	894-902 bp	1.0	55.8