Hotspot

ndhK-ndhC

Genome · 47293-47631 · 339 bp

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Pi 0.0268

MarkerSeek score 56.50

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: Genome
length_bp: 339
variable_sites: 9
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	ACTGAACATCCCCCAGTAAT	CCAAGGGCAATGAGTTTCGA	45.0	50.0	55.8	58.5	406-409 bp	1.0	61.0
ndhK-ndhC_p2	CTGAACATCCCCCAGTAATTCA	CCAAGGGCAATGAGTTTCGA	45.5	50.0	57.8	58.5	405-408 bp	1.0	60.7
ndhK-ndhC_p3	ACTGAACATCCCCCAGTAATTC	CCAAGGGCAATGAGTTTCGA	45.5	50.0	57.8	58.5	406-409 bp	1.0	60.7
ndhK-ndhC_p4	ACTGAACATCCCCCAGTAAT	AGGGCAATGAGTTTCGATGT	45.0	45.0	55.8	57.5	403-406 bp	1.0	60.7
ndhK-ndhC_p5	CTGAACATCCCCCAGTAATTCA	AGGGCAATGAGTTTCGATGT	45.5	45.0	57.8	57.5	402-405 bp	1.0	60.5