Hotspot

trnF-GAA-ndhJ

Genome · 45972-46666 · 695 bp

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Pi 0.0118

MarkerSeek score 67.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 695
variable_sites: 8
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ACTGAATGCCTGAACTGTGT	55.0	45.0	58.0	57.4	832-833 bp	1.0	77.0
trnF-GAA-ndhJ_p2	TGGTGCATCAGTAATGGTCG	ACTGAATGCCTGAACTGTGT	50.0	45.0	58.0	57.4	851-852 bp	1.0	76.8
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ACTGAATGCCTGAACTGTGT	50.0	45.0	58.1	57.4	796-797 bp	1.0	76.7
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ACTGAATGCCTGAACTGTGT	55.0	45.0	57.9	57.4	820-821 bp	1.0	76.0
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	AACTGAATGCCTGAACTGTGT	55.0	42.9	58.0	58.1	833-834 bp	1.0	73.7