Hotspot

psbM-trnD-GUC

Genome · 28750-29800 · 1051 bp

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Pi 0.00961

MarkerSeek score 63.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: Genome
length_bp: 1051
variable_sites: 10
pi: 0.00961
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1152-1162 bp	1.0	59.7
psbM-trnD-GUC_p2	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1159-1169 bp	1.0	56.6
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	1153-1163 bp	1.0	55.6
psbM-trnD-GUC_p4	ACGCAGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1166-1176 bp	1.0	55.2
psbM-trnD-GUC_p5	GGAACTAGAATGAACAGTGCAG	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.1	58.5	1158-1168 bp	1.0	54.1