Hotspot

atpH-atpI

Genome · 12915-13634 · 720 bp

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Pi 0.0183

MarkerSeek score 60.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 720
variable_sites: 13
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	AATCCGTGGAGGGTCATCAT	50.0	50.0	58.1	58.5	781-807 bp	1.0	77.7
atpH-atpI_p2	GCAGCAATAACAGAAGCAGC	ATCCGTGGAGGGTCATCATT	50.0	50.0	58.1	58.5	780-806 bp	1.0	77.7
atpH-atpI_p3	GCAGCAATAACAGAAGCAGC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.1	58.5	814-840 bp	1.0	77.3
atpH-atpI_p4	CGCAGCAATAACAGAAGCAG	AATCCGTGGAGGGTCATCAT	50.0	50.0	57.8	58.5	782-808 bp	1.0	77.1
atpH-atpI_p5	CGCAGCAATAACAGAAGCAG	ATCCGTGGAGGGTCATCATT	50.0	50.0	57.8	58.5	781-807 bp	1.0	77.1