Hotspot

atpF-atpH

Genome · 12272-12668 · 397 bp

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Pi 0.0207

MarkerSeek score 66.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 397
variable_sites: 8
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGATGGCCAATGACCTAAGG	GGAGGGGAAGATACGAGGTA	55.0	55.0	58.0	57.3	547-557 bp	1.0	77.1
atpF-atpH_p2	CGGATGGCCAATGACCTAAG	GGAGGGGAAGATACGAGGTA	55.0	55.0	58.4	57.3	548-558 bp	1.0	74.0
atpF-atpH_p3	TATTAAACCCGAAACCCCCG	GGAGGGGAAGATACGAGGTA	50.0	55.0	57.6	57.3	569-579 bp	1.0	73.8
atpF-atpH_p4	GGATGGCCAATGACCTAAGG	GGAGGGGAAGATACGAGGT	55.0	57.9	58.0	57.5	547-557 bp	1.0	70.2
atpF-atpH_p5	GGATGGCCAATGACCTAAGG	CGAAGCGGAGGGGAAGATA	55.0	57.9	58.0	58.6	553-563 bp	1.0	69.9