Hotspot

trnK-UUU-rps16

Genome · 3864-4241 · 378 bp

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Pi 0.0219

MarkerSeek score 55.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: Genome
length_bp: 378
variable_sites: 8
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCTTTCAGGATCAGTCGTG	TCCTGAAACAAGGAAAGCCC	55.0	50.0	58.0	58.0	1016-1019 bp	1.0	82.1
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	TCCTGAAACAAGGAAAGCCC	55.0	50.0	58.0	58.0	1015-1018 bp	1.0	82.1
trnK-UUU-rps16_p3	ACCCAACCGCTCTTTTGATT	AAAAAGGCGCTCAACCTACA	45.0	45.0	58.0	58.0	907-916 bp	1.0	82.1
trnK-UUU-rps16_p4	AACCCAACCGCTCTTTTGAT	TCCTGAAACAAGGAAAGCCC	45.0	50.0	58.0	58.0	1360-1363 bp	1.0	81.7
trnK-UUU-rps16_p5	ACCCAACCGCTCTTTTGATT	TCCTGAAACAAGGAAAGCCC	45.0	50.0	58.0	58.0	1359-1362 bp	1.0	81.7