Hotspot

rpl16

LSC · 82106-83874 · 1769 bp

Back to Schoenorchis

Pi 0.0255

MarkerSeek score 64.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1769
variable_sites: 42
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTGGGCACTGCTTCTTTGA	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2331-2413 bp	1.0	84.9
rpl16_p2	TGGGCACTGCTTCTTTGATT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2329-2411 bp	1.0	84.9
rpl16_p3	TTGGGCACTGCTTCTTTGAT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2330-2412 bp	1.0	84.9
rpl16_p4	TGGGCACTGCTTCTTTGATT	AAGGACAAACACGGGGAATC	45.0	50.0	58.0	58.1	2558-2640 bp	1.0	84.8
rpl16_p5	TGGGCACTGCTTCTTTGATT	GAAGGACAAACACGGGGAAT	45.0	50.0	58.0	58.1	2559-2641 bp	1.0	84.8