Hotspot

ndhD

SSC · 110695-111610 · 916 bp

Back to Schoenorchis

Pi 0.0304

MarkerSeek score 58.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 916
variable_sites: 18
pi: 0.0304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGCATTCTTTCTGGAGCAG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	1428-1696 bp	1.0	84.4
ndhD_p2	AGGCATTCTTTCTGGAGCAG	GCACGGGCTTTTCTGGTATA	50.0	50.0	57.9	58.0	1245-1513 bp	1.0	84.4
ndhD_p3	GGCATTCTTTCTGGAGCAGT	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.2	58.0	1427-1695 bp	1.0	84.1
ndhD_p4	GGCATTCTTTCTGGAGCAGT	GCACGGGCTTTTCTGGTATA	50.0	50.0	58.2	58.0	1244-1512 bp	1.0	84.1
ndhD_p5	TCTGGAGCAGTATGGGCTAA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	1418-1686 bp	1.0	84.0