Hotspot

ndhI-rps15

SSC · 113674-113776 · 103 bp

Back to Schoenorchis

Pi 0.0737

MarkerSeek score 72.90

Primer available yes

Label ndhI-rps15

Feature Metadata

label_name: ndhI-rps15
feature_type: igs
region: SSC
length_bp: 103
variable_sites: 7
pi: 0.0737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-rps15_p1	AAATAAGGAACTGCGTGGCA	TTCTAGGAAAACGTCAACGA	45.0	40.0	58.1	54.7	471-480 bp	1.0	57.3
ndhI-rps15_p2	TGCGTGGCAGCTACAAAATA	TTCTAGGAAAACGTCAACGA	45.0	40.0	58.2	54.7	460-469 bp	1.0	56.5
ndhI-rps15_p3	CAAATAAGGAACTGCGTGGC	TTCTAGGAAAACGTCAACGA	50.0	40.0	57.7	54.7	472-481 bp	1.0	56.0
ndhI-rps15_p4	CCGTTCATAAACAGCACAGC	TTCTAGGAAAACGTCAACGA	50.0	40.0	57.8	54.7	521-530 bp	1.0	55.8
ndhI-rps15_p5	AAATAAGGAACTGCGTGGCA	TCCTGATCGGTAAAATTCACAGA	45.0	39.1	58.1	57.6	687-696 bp	1.0	55.4