Hotspot

psaC-ndhE

SSC · 111973-112580 · 608 bp

Back to Schoenorchis

Pi 0.0445

MarkerSeek score 74.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 608
variable_sites: 25
pi: 0.0445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGACAAGCGGATTCACATCT	ACAAGTCGAAGCATGGCTAG	50.0	50.0	58.0	58.0	1025 bp	0.5	59.6
psaC-ndhE_p2	TATACCAGAAAAGCCCGTGC	ACAAGTCGAAGCATGGCTAG	50.0	50.0	58.0	58.0	1208 bp	0.5	59.6
psaC-ndhE_p3	AGCTCGAACACATTGGGTAC	ACAAGTCGAAGCATGGCTAG	50.0	50.0	57.9	58.0	909 bp	0.5	59.1
psaC-ndhE_p4	GCTCGAACACATTGGGTACA	ACAAGTCGAAGCATGGCTAG	50.0	50.0	58.2	58.0	908 bp	0.5	58.3
psaC-ndhE_p5	TTGGACAAGCGGATTCACAT	ACAAGTCGAAGCATGGCTAG	45.0	50.0	57.8	58.0	1027 bp	0.5	58.3