Hotspot

psbB-psbT

LSC · 74394-74784 · 391 bp

Back to Schoenorchis

Pi 0.0421

MarkerSeek score 75.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 15
pi: 0.0421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	585-586 bp	1.0	72.7
psbB-psbT_p2	TCAGAGATGTTTTTGCCGGT	GGAACTTTAGGTGGTTCTCGA	45.0	47.6	57.7	57.3	584-585 bp	1.0	70.5
psbB-psbT_p3	GCCAGAACCTTGTTCAGAGA	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	598-599 bp	1.0	70.4
psbB-psbT_p4	GCTCAAGTGGAATTTGGAGC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.4	57.6	547-548 bp	1.0	69.9
psbB-psbT_p5	GCCAGAACCTTGTTCAGAGA	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.4	57.3	597-598 bp	1.0	68.2