Hotspot

psbL-psbF

LSC · 64479-64500 · 22 bp

Back to Schoenorchis

Pi 0.0455

MarkerSeek score 73.80

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 1
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGGGTTTGATTGTGTCTTTG	TCAGCAATGCAGTTCATCCA	42.9	45.0	57.0	57.8	80 bp	0.5	42.5
psbL-psbF_p2	TCATTCGGGTTTGATTGTGTC	TCAGCAATGCAGTTCATCCA	42.9	45.0	56.8	57.8	84 bp	0.5	40.9
psbL-psbF_p3	CATTCGGGTTTGATTGTGTCT	TCAGCAATGCAGTTCATCCA	42.9	45.0	56.8	57.8	83 bp	0.5	40.8
psbL-psbF_p4	CGGGTTTGATTGTGTCTTTGA	GGACTGGCTGTACCTACTGT	42.9	55.0	57.0	58.4	118 bp	0.5	40.6
psbL-psbF_p5	TCGGGTTTGATTGTGTCTTTG	GGACTGGCTGTACCTACTGT	42.9	55.0	57.0	58.4	119 bp	0.5	40.6