Hotspot

atpB-rbcL

LSC · 54021-54931 · 911 bp

Back to Schoenorchis

Pi 0.0556

MarkerSeek score 77.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 49
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTTTCCTCCACGGCGATAG	GTCCACACAGTTGTCCATGT	50.0	50.0	58.0	58.0	1652-1677 bp	1.0	83.7
atpB-rbcL_p2	TTTTTCCTCCACGGCGATAG	GGGGACGACCATACTTGTTC	50.0	55.0	58.0	58.0	1945-1970 bp	1.0	83.2
atpB-rbcL_p3	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2095-2120 bp	1.0	83.1
atpB-rbcL_p4	ATACGCCTCCATGAGCTTTG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2182-2207 bp	1.0	82.9
atpB-rbcL_p5	ATACGCCTCCATGAGCTTTG	GGGGACGACCATACTTGTTC	50.0	55.0	58.0	58.0	2032-2057 bp	1.0	82.9