Hotspot

rpoC1-rpoB

LSC · 23580-23605 · 26 bp

Back to Schoenorchis

Pi 0.115

MarkerSeek score 74.80

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 3
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GGGAAACTGGTCCAATTCGA	CCAGATGGATAGGAAGGAAGC	50.0	52.4	57.8	57.6	100 bp	1.0	69.5
rpoC1-rpoB_p2	TTGTTGAGGGGAAACTGGTC	CCAGATGGATAGGAAGGAAGC	50.0	52.4	57.6	57.6	108 bp	1.0	68.0
rpoC1-rpoB_p3	TGAGGGGAAACTGGTCCAAT	CCAGATGGATAGGAAGGAAGC	50.0	52.4	58.5	57.6	104 bp	1.0	66.6
rpoC1-rpoB_p4	AGGGGAAACTGGTCCAATTC	CCAGATGGATAGGAAGGAAGC	50.0	52.4	57.4	57.6	102 bp	1.0	66.2
rpoC1-rpoB_p5	GAGGGGAAACTGGTCCAATT	CCAGATGGATAGGAAGGAAGC	50.0	52.4	57.4	57.6	103 bp	1.0	66.2