Hotspot

rps16-trnQ-UUG

LSC · 5993-6898 · 906 bp

Back to Schoenorchis

Pi 0.0563

MarkerSeek score 77.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 49
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	976-979 bp	1.0	85.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TAGCGGCACCATGTCCTATA	45.0	50.0	58.1	58.0	1110-1113 bp	1.0	85.2
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	TAGCGGCACCATGTCCTATA	50.0	50.0	58.2	58.0	1121-1124 bp	1.0	84.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.1	57.9	1569-1602 bp	1.0	83.4
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.2	57.9	1580-1613 bp	1.0	82.9