Hotspot

petB

LSC · 74731-76124 · 1394 bp

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Pi 0.0118

MarkerSeek score 55.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1394
variable_sites: 30
pi: 0.0118
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AACGCGAATTTCTGGCCTAT	GTGCTTTCTGGGTTGTCTCA	45.0	50.0	58.0	58.0	2109-2119 bp	0.8	73.6
petB_p2	ACGCGAATTTCTGGCCTATT	GTGCTTTCTGGGTTGTCTCA	45.0	50.0	58.0	58.0	2108-2118 bp	0.8	73.6
petB_p3	AACGCGAATTTCTGGCCTAT	AGATCTTACGGAGCCTCCTC	45.0	55.0	58.0	58.0	1845 bp	0.4	55.7
petB_p4	ACGCGAATTTCTGGCCTATT	AGATCTTACGGAGCCTCCTC	45.0	55.0	58.0	58.0	1844 bp	0.4	55.7
petB_p5	GAAATCGGGTCCAAGACGAA	AGATCTTACGGAGCCTCCTC	50.0	55.0	57.9	58.0	2091 bp	0.4	55.2