Hotspot

ndhG-ndhI

SSC · 123161-123486 · 326 bp

Back to Schnabelia

Pi 0.0282

MarkerSeek score 47.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 326
variable_sites: 20
pi: 0.0282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	GTCGCTTACCGGCATCAATA	50.0	50.0	58.0	58.1	460-463 bp	1.0	85.1
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	TTTGCCGAGACTGAGAATGG	50.0	50.0	58.0	57.9	1079-1089 bp	1.0	83.0
ndhG-ndhI_p3	AAGACCCGATCCCAGAAAGA	CAAGATGGACTTTGCCGAGA	50.0	50.0	58.0	57.9	1089-1099 bp	1.0	82.9
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	AAGATGGACTTTGCCGAGAC	50.0	50.0	58.0	57.9	1088-1098 bp	1.0	82.9
ndhG-ndhI_p5	AAGACCCGATCCCAGAAAGA	ACAAACAATACGAGCTGCCA	50.0	45.0	58.0	58.1	849-852 bp	1.0	82.9