Hotspot

psbE-petL

LSC · 64857-65770 · 914 bp

Back to Schnabelia

Pi 0.0175

MarkerSeek score 59.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 30
pi: 0.0175
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1099-1106 bp	1.0	74.9
psbE-petL_p2	ACTGACGAATAACCAACCCG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1112-1119 bp	1.0	74.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	1108-1115 bp	1.0	73.9
psbE-petL_p4	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1107-1114 bp	1.0	73.8
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.2	57.1	1101-1108 bp	1.0	73.4