Hotspot

rbcL-accD

LSC · 57102-57776 · 675 bp

Back to Schnabelia

Pi 0.016

MarkerSeek score 60.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 22
pi: 0.016
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ATAGGATCCCAAGTACCGGG	55.0	55.0	58.0	58.0	1324-1344 bp	1.0	83.4
rbcL-accD_p2	TCTTCACATTCACCGTGCAA	ATAGGATCCCAAGTACCGGG	45.0	55.0	58.0	58.0	1793-1813 bp	1.0	82.7
rbcL-accD_p3	TTCTTCACATTCACCGTGCA	ATAGGATCCCAAGTACCGGG	45.0	55.0	58.0	58.0	1794-1814 bp	1.0	82.7
rbcL-accD_p4	CCGCAAATACTACCTTGGCT	ATAGGATCCCAAGTACCGGG	50.0	55.0	58.0	58.0	1839-1858 bp	0.6	66.0
rbcL-accD_p5	CCGCAAATACTACCTTGGCT	GATCCCCATCCGTTTTCTCC	50.0	55.0	58.0	58.0	1913-1932 bp	0.6	65.9