Hotspot

atpA-atpF

LSC · 12313-12410 · 98 bp

Back to Schnabelia

Pi 0.052

MarkerSeek score 69.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 98
variable_sites: 11
pi: 0.052
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATACGAGCAATACCATCGCC	AGCCATTCAACAGCTGGAAA	50.0	45.0	57.9	58.0	552-572 bp	1.0	84.6
atpA-atpF_p2	AACGCCGGGAAATAATACCC	AGCCATTCAACAGCTGGAAA	50.0	45.0	58.0	58.0	851-871 bp	1.0	83.3
atpA-atpF_p3	AACGCCGGGAAATAATACCC	GTTTGGTTCGGGAAGGGATT	50.0	50.0	58.0	58.1	985-1005 bp	1.0	82.3
atpA-atpF_p4	ACTGCTGTTTTACCGGTCTG	AGCCATTCAACAGCTGGAAA	50.0	45.0	58.1	58.0	963-983 bp	1.0	82.2
atpA-atpF_p5	CTGCTGTTTTACCGGTCTGT	AGCCATTCAACAGCTGGAAA	50.0	45.0	58.1	58.0	962-982 bp	1.0	82.2