Hotspot

trnK-UUU-rps16

LSC · 4331-5280 · 950 bp

Back to Schnabelia

Pi 0.0203
MarkerSeek score 65.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
950
variable_sites
36
pi
0.0203
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAACTTCCGCCTAATGAA 50.0 45.0 58.2 56.5 1010-1024 bp 1.0 71.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATGAA 50.0 45.0 58.2 56.5 1009-1023 bp 1.0 71.9
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC AGGAACTTCCGCCTAATGAA 50.0 45.0 58.3 56.5 1024-1038 bp 1.0 71.1
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AGGAACTTCCGCCTAATGAA 50.0 45.0 58.3 56.5 1019-1033 bp 1.0 71.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATGAAA 50.0 42.9 58.2 57.3 1009-1023 bp 1.0 70.0