Hotspot

rps3

LSC · 81686-82405 · 720 bp

Back to Schizostachyum

Pi 0.00278

MarkerSeek score 60.40

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 720
variable_sites: 3
pi: 0.00278
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	ACAGAATGGGAAGACAAGGC	TCCATCTAAGATTTCGATTCCCA	50.0	39.1	57.8	57.1	789 bp	1.0	47.6
rps3_p2	ACAGAATGGGAAGACAAGGC	TCCATCTAAGATTTCGATTCCCAG	50.0	41.7	57.8	58.1	789 bp	1.0	45.5
rps3_p3	CAGAATGGGAAGACAAGGCT	TCCATCTAAGATTTCGATTCCCA	50.0	39.1	57.5	57.1	788 bp	1.0	45.2
rps3_p4	ACAGAATGGGAAGACAAGGC	CCATCTAAGATTTCGATTCCCAG	50.0	43.5	57.8	56.8	788 bp	1.0	45.1
rps3_p5	ACAGAATGGGAAGACAAGGC	CCATCTAAGATTTCGATTCCCA	50.0	40.9	57.8	55.7	788 bp	1.0	44.0