Hotspot

ndhG

SSC · 112953-113483 · 531 bp

Back to Schizostachyum

Pi 0.00126

MarkerSeek score 34.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AAGAATTTTGACTCATTCTAAGT	TCCTTTTTCCTTCCTTGAATCT	26.1	36.4	52.0	55.2	637 bp	1.0	40.1
ndhG_p2	TGAAATTCTAAGAATTTTGACTCA	TCCTTTTTCCTTCCTTGAATCT	25.0	36.4	52.9	55.2	646 bp	1.0	40.1
ndhG_p3	AAGAATTTTGACTCATTCTAAGT	TCCTTTTTCCTTCCTTGAATCTT	26.1	34.8	52.0	56.0	637 bp	1.0	40.1
ndhG_p4	AAGAATTTTGACTCATTCTAAGT	TTCCTTTTTCCTTCCTTGAATCT	26.1	34.8	52.0	56.0	638 bp	1.0	40.1
ndhG_p5	TCTAAGAATTTTGACTCATTCTAAGT	TCCTTTTTCCTTCCTTGAATCT	26.9	36.4	54.7	55.2	640 bp	1.0	40.1