Hotspot

ndhD

SSC · 110055-111557 · 1503 bp

Back to Schizostachyum

Pi 0.0031

MarkerSeek score 60.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 7
pi: 0.0031
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGAGTATAGAGCGGACTAGT	CGGGCTTTTCTGGTCAAAAC	45.0	50.0	53.9	57.9	1585-1586 bp	1.0	46.5
ndhD_p2	AGAGTATAGAGCGGACTAGT	GGGCTTTTCTGGTCAAAACG	45.0	50.0	53.9	57.9	1584-1585 bp	1.0	46.5
ndhD_p3	AGAGTATAGAGCGGACTAGT	ACGGGCTTTTCTGGTCAAAA	45.0	45.0	53.9	58.2	1586-1587 bp	1.0	45.8
ndhD_p4	AGAGTATAGAGCGGACTAGTT	CGGGCTTTTCTGGTCAAAAC	42.9	50.0	54.8	57.9	1585-1586 bp	1.0	45.6
ndhD_p5	AGAGTATAGAGCGGACTAGTT	GGGCTTTTCTGGTCAAAACG	42.9	50.0	54.8	57.9	1584-1585 bp	1.0	45.6