Hotspot

accD-psaI

LSC · 58656-59319 · 664 bp

Back to Schizostachyum

Pi 0.00502

MarkerSeek score 64.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 664
variable_sites: 5
pi: 0.00502
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACCTTTACCGCGCATTAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.7	58.0	748-761 bp	1.0	76.1
accD-psaI_p2	CCACCTTTACCGCGCATTAT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.7	57.9	754-767 bp	1.0	75.5
accD-psaI_p3	ATTTTTCCACCTTTACCGCG	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.0	58.0	754-767 bp	1.0	73.7
accD-psaI_p4	ATTTTTCCACCTTTACCGCG	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.0	57.9	760-773 bp	1.0	73.0
accD-psaI_p5	CCTTTACCGCGCATTATTGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.9	58.0	745-758 bp	1.0	72.7