Hotspot

rbcL-accD

LSC · 58023-58568 · 546 bp

Back to Schizostachyum

Pi 0.0244

MarkerSeek score 72.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 19
pi: 0.0244
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGCAGCTTGTGAAGTATGGA	CCTGTATACGCGGTCCTCTA	50.0	55.0	58.3	58.1	680-744 bp	1.0	83.0
rbcL-accD_p2	CGCAGCTTGTGAAGTATGGA	ATACGCGGTCCTCTATCCTC	50.0	55.0	58.3	58.2	675-739 bp	1.0	82.5
rbcL-accD_p3	GGCGATCAAATTCGAGTTCG	CCTGTATACGCGGTCCTCTA	50.0	55.0	57.6	58.1	659-723 bp	1.0	82.5
rbcL-accD_p4	CGCAGCTTGTGAAGTATGGA	GCTCATACAATAATGCGCGG	50.0	50.0	58.3	57.8	650-714 bp	1.0	82.2
rbcL-accD_p5	TGGAAGGCGATCAAATTCGA	CCTGTATACGCGGTCCTCTA	45.0	55.0	57.6	58.1	664-728 bp	1.0	82.1