Hotspot

NQW19_pgp044

Genome · 56447-58456 · 2010 bp

Back to Schizaea

Pi 0.118

MarkerSeek score 83.30

Primer available yes

Label NQW19_pgp044

Feature Metadata

label_name: NQW19_pgp044
feature_type: gene
region: Genome
length_bp: 2010
variable_sites: 652
pi: 0.118
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp044_p1	CCGATTGTAACCACGAACCT	CGAACCGAGGTGCAGAATAA	50.0	50.0	57.9	58.0	2938-2976 bp	0.625	73.5
NQW19_pgp044_p2	GCGTCACACATACACCCTAG	CGAACCGAGGTGCAGAATAA	55.0	50.0	58.1	58.0	2817-2854 bp	0.5	66.1
NQW19_pgp044_p3	TTCTCTGCAGCAGTAGGAGT	CGAACCGAGGTGCAGAATAA	50.0	50.0	58.1	58.0	2494-2502 bp	0.25	52.8
NQW19_pgp044_p4	GGGGGATATTTCTCTGCAGC	CGAACCGAGGTGCAGAATAA	55.0	50.0	58.1	58.0	2503-2511 bp	0.25	52.6
NQW19_pgp044_p5	TTCTCTGCAGCAGTAGGAGT	ACCTCCCCAAGACTTCGTTA	50.0	50.0	58.1	58.0	2793-2801 bp	0.25	52.4