Hotspot

NQW19_pgp031

Genome · 68306-69612 · 1307 bp

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Pi 0.124

MarkerSeek score 84.30

Primer available yes

Label NQW19_pgp031

Feature Metadata

label_name: NQW19_pgp031
feature_type: gene
region: Genome
length_bp: 1307
variable_sites: 450
pi: 0.124
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp031_p1	CAATCCATAAGGGGCAGCAT	AACCGGTAATGAGGGGGTTA	50.0	50.0	58.0	58.0	1724 bp	0.125	44.7
NQW19_pgp031_p2	GGTCAATCCATAAGGGGCAG	AACCGGTAATGAGGGGGTTA	55.0	50.0	58.0	58.0	1727 bp	0.125	44.7
NQW19_pgp031_p3	CAATCCATAAGGGGCAGCAT	GAATGGGCCAGAGAAGGAAG	50.0	55.0	58.0	58.0	1657 bp	0.125	44.6
NQW19_pgp031_p4	CAATCCATAAGGGGCAGCAT	GGGACGCCTTAATGGTAGTG	50.0	55.0	58.0	58.1	1691 bp	0.125	44.6
NQW19_pgp031_p5	AGGGGCAGCATATCTTTTCG	AACCGGTAATGAGGGGGTTA	50.0	50.0	58.0	58.0	1715 bp	0.125	44.5