Hotspot

NQW19_pgp044-NQW19_pgp043

Genome · 58457-58972 · 516 bp

Back to Schizaea

Pi 0.149

MarkerSeek score 83.40

Primer available yes

Label NQW19_pgp044-NQW19_pgp043

Feature Metadata

label_name: NQW19_pgp044-NQW19_pgp043
feature_type: igs
region: Genome
length_bp: 516
variable_sites: 207
pi: 0.149
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp044-NQW19_pgp043_p1	CTCGTTTTCTACTTCGGGGG	ACCTCCCCAAGACTTCGTTA	55.0	50.0	58.0	58.0	1226-1242 bp	0.625	72.8
NQW19_pgp044-NQW19_pgp043_p2	TTAATTGCGACGAGACAGGG	ACCTCCCCAAGACTTCGTTA	50.0	50.0	58.0	58.0	914-919 bp	0.5	63.7
NQW19_pgp044-NQW19_pgp043_p3	ACTAAACGCCCCAACACATT	ACCTCCCCAAGACTTCGTTA	45.0	50.0	58.0	58.0	855 bp	0.5	63.4
NQW19_pgp044-NQW19_pgp043_p4	CTCGTTTTCTACTTCGGGGG	GTTCCCGGTGATACTCCAAC	55.0	55.0	58.0	58.0	1256-1260 bp	0.375	58.8
NQW19_pgp044-NQW19_pgp043_p5	TTAATTGCGACGAGACAGGG	GTTCCCGGTGATACTCCAAC	50.0	55.0	58.0	58.0	932-937 bp	0.375	58.2