Hotspot

NQW19_pgp074-NQW19_pgt004

Genome · 19052-20419 · 1368 bp

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Pi 0.233

MarkerSeek score 72.20

Primer available yes

Label NQW19_pgp074-NQW19_pgt004

Feature Metadata

label_name: NQW19_pgp074-NQW19_pgt004
feature_type: igs
region: Genome
length_bp: 1368
variable_sites: 655
pi: 0.233
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp074-NQW19_pgt004_p1	AACAAAGCGGTGGAATCCTT	GCAGCCCAGGTTATGCTAAT	45.0	50.0	58.0	58.0	1992-2186 bp	0.5	67.4
NQW19_pgp074-NQW19_pgt004_p2	AACAAAGCGGTGGAATCCTT	ATGACGAGCCAGATCCCATA	45.0	50.0	58.0	58.0	2094-2288 bp	0.5	67.3
NQW19_pgp074-NQW19_pgt004_p3	AACAAAGCGGTGGAATCCTT	TATGACGAGCCAGATCCCAT	45.0	50.0	58.0	58.0	2110-2127 bp	0.25	54.0
NQW19_pgp074-NQW19_pgt004_p4	AACAAAGCGGTGGAATCCTT	TCTCCCTGCCTCGCAATATA	45.0	50.0	58.0	58.0	1654 bp	0.125	44.9
NQW19_pgp074-NQW19_pgt004_p5	GAACCCCCTCTATGATCCGA	TCTCCCTGCCTCGCAATATA	55.0	50.0	58.0	58.0	2004 bp	0.125	44.8