Hotspot

NQW19_pgt001-NQW19_pgp082

Genome · 778-1366 · 589 bp

Back to Schizaea

Pi 0.275
MarkerSeek score 72.50
Primer available yes
Label NQW19_pgt001-NQW19_pgp082

Feature Metadata

label_name
NQW19_pgt001-NQW19_pgp082
feature_type
igs
region
Genome
length_bp
589
variable_sites
327
pi
0.275
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.86
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
NQW19_pgt001-NQW19_pgp082_p1 CGGTTGTAGATGTGATGCCA ATCTACCCTCCGCTTAGACC 50.0 55.0 58.0 58.0 1450-1464 bp 0.5 69.6
NQW19_pgt001-NQW19_pgp082_p2 CCAGAAGCTTACGCCATTCT ATCTACCCTCCGCTTAGACC 50.0 55.0 58.0 58.0 1475-1489 bp 0.5 69.5
NQW19_pgt001-NQW19_pgp082_p3 GTTTCTCTCGCAAAACTGCC ATCTACCCTCCGCTTAGACC 50.0 55.0 58.0 58.0 1493-1507 bp 0.5 69.4
NQW19_pgt001-NQW19_pgp082_p4 CTAGCTTTTGTGTGGCAAGC ATCTACCCTCCGCTTAGACC 50.0 55.0 57.9 58.0 1406-1420 bp 0.5 69.4
NQW19_pgt001-NQW19_pgp082_p5 AGAAGCTTACGCCATTCTCG ATCTACCCTCCGCTTAGACC 50.0 55.0 58.1 58.0 1473-1487 bp 0.5 69.1