primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgt001-NQW19_pgp082_p1	NQW19_pgt001-NQW19_pgp082	1	CGGTTGTAGATGTGATGCCA	ATCTACCCTCCGCTTAGACC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.024542	778	1366	1450-1464	0.500000	69.6
NQW19_pgt001-NQW19_pgp082_p2	NQW19_pgt001-NQW19_pgp082	2	CCAGAAGCTTACGCCATTCT	ATCTACCCTCCGCTTAGACC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.031616	778	1366	1475-1489	0.500000	69.5
NQW19_pgt001-NQW19_pgp082_p3	NQW19_pgt001-NQW19_pgp082	3	GTTTCTCTCGCAAAACTGCC	ATCTACCCTCCGCTTAGACC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.039718	778	1366	1493-1507	0.500000	69.4
NQW19_pgt001-NQW19_pgp082_p4	NQW19_pgt001-NQW19_pgp082	4	CTAGCTTTTGTGTGGCAAGC	ATCTACCCTCCGCTTAGACC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.057872	778	1366	1406-1420	0.500000	69.4
NQW19_pgt001-NQW19_pgp082_p5	NQW19_pgt001-NQW19_pgp082	5	AGAAGCTTACGCCATTCTCG	ATCTACCCTCCGCTTAGACC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.073883	778	1366	1473-1487	0.500000	69.1
NQW19_pgp074-NQW19_pgt004_p1	NQW19_pgp074-NQW19_pgt004	1	AACAAAGCGGTGGAATCCTT	GCAGCCCAGGTTATGCTAAT	20	20	45.0	50.0	58.0	58.0	0.0	1.4	0.017741	19052	20419	1992-2186	0.500000	67.4
NQW19_pgp074-NQW19_pgt004_p2	NQW19_pgp074-NQW19_pgt004	2	AACAAAGCGGTGGAATCCTT	ATGACGAGCCAGATCCCATA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.020080	19052	20419	2094-2288	0.500000	67.3
NQW19_pgp074-NQW19_pgt004_p3	NQW19_pgp074-NQW19_pgt004	3	AACAAAGCGGTGGAATCCTT	TATGACGAGCCAGATCCCAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.020080	19052	20419	2110-2127	0.250000	54.0
NQW19_pgp074-NQW19_pgt004_p4	NQW19_pgp074-NQW19_pgt004	4	AACAAAGCGGTGGAATCCTT	TCTCCCTGCCTCGCAATATA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.014305	19052	20419	1654-1654	0.125000	44.9
NQW19_pgp074-NQW19_pgt004_p5	NQW19_pgp074-NQW19_pgt004	5	GAACCCCCTCTATGATCCGA	TCTCCCTGCCTCGCAATATA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.020804	19052	20419	2004-2004	0.125000	44.8
NQW19_pgp062-NQW19_pgp061_p1	NQW19_pgp062-NQW19_pgp061	1	AGAACCGTTTTTCCCACTCC	CGCGGAGACACTCATAAACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032145	42388	43023	1746-1755	0.625000	72.5
NQW19_pgp062-NQW19_pgp061_p2	NQW19_pgp062-NQW19_pgp061	2	CGTAGACTAGCGCCACTTTT	CGCGGAGACACTCATAAACA	20	20	50.0	50.0	58.0	58.0	2.3	0.0	0.017031	42388	43023	1913-1922	0.625000	72.3
NQW19_pgp062-NQW19_pgp061_p3	NQW19_pgp062-NQW19_pgp061	3	TTGAGCCAACTCTCATACGC	CGCGGAGACACTCATAAACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019346	42388	43023	1958-1967	0.625000	72.1
NQW19_pgp062-NQW19_pgp061_p4	NQW19_pgp062-NQW19_pgp061	4	CCCATGAGCCTTGGCAATAT	CGCGGAGACACTCATAAACA	20	20	50.0	50.0	58.0	58.0	14.5	0.0	0.014571	42388	43023	1786-1795	0.500000	67.1
NQW19_pgp062-NQW19_pgp061_p5	NQW19_pgp062-NQW19_pgp061	5	CCCATGAGCCTTGGCAATAT	ATCTCGTTCAACCTGGATGC	20	20	50.0	50.0	58.0	58.0	14.5	0.0	0.035208	42388	43023	1683-1692	0.375000	57.9
NQW19_pgp046-NQW19_pgp045_p1	NQW19_pgp046-NQW19_pgp045	1	AACAATCTCGAATGAGCCCC	GTGTCATGGAAGCAGAGGAG	20	20	50.0	55.0	58.0	58.0	0.1	0.0	0.065478	55265	56059	1438-1477	0.750000	80.4
NQW19_pgp046-NQW19_pgp045_p2	NQW19_pgp046-NQW19_pgp045	2	TTTACTCTCTGCTGGTTCGC	GTGTCATGGAAGCAGAGGAG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.101641	55265	56059	1465-1504	0.750000	80.1
NQW19_pgp046-NQW19_pgp045_p3	NQW19_pgp046-NQW19_pgp045	3	CTCGGTATCCAAAATGGCCA	GTGTCATGGAAGCAGAGGAG	20	20	50.0	55.0	57.9	58.0	8.6	0.0	0.072690	55265	56059	1665-1694	0.625000	72.5
NQW19_pgp046-NQW19_pgp045_p4	NQW19_pgp046-NQW19_pgp045	4	AACAATCTCGAATGAGCCCC	AATCCATGTTCGAAGGACGG	20	20	50.0	50.0	58.0	58.0	0.1	6.8	0.068298	55265	56059	1428-1428	0.125000	44.5
NQW19_pgp046-NQW19_pgp045_p5	NQW19_pgp046-NQW19_pgp045	5	CTCGGTATCCAAAATGGCCA	AATCCATGTTCGAAGGACGG	20	20	50.0	50.0	57.9	58.0	8.6	6.8	0.075510	55265	56059	1645-1645	0.125000	44.4
NQW19_pgp045_p1	NQW19_pgp045	1	ATCGACGCGTGATAAACCAA	GGAAGACAAACTTTCGCACG	20	20	45.0	50.0	58.0	58.0	20.7	0.0	0.019669	128397	128646	739-739	0.125000	44.8
NQW19_pgp045_p2	NQW19_pgp045	2	CAATCCTCCTGCAAATCGGA	GGAAGACAAACTTTCGCACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061403	128397	128646	1083-1083	0.125000	44.5
NQW19_pgp045_p3	NQW19_pgp045	3	TCCAATCCTCCTGCAAATCG	GGAAGACAAACTTTCGCACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061403	128397	128646	1085-1085	0.125000	44.5
NQW19_pgp045_p4	NQW19_pgp045	4	AGGCTTACTGCATGGCTTTT	GGAAGACAAACTTTCGCACG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.089849	128397	128646	913-913	0.125000	44.3
NQW19_pgp045_p5	NQW19_pgp045	5	ATCGACGCGTGATAAACCAA	GAGAACCTGGATAACCCCCT	20	20	45.0	55.0	58.0	58.1	20.7	0.0	0.106252	128397	128646	1065-1065	0.125000	44.1
NQW19_pgp044_p1	NQW19_pgp044	1	CCGATTGTAACCACGAACCT	CGAACCGAGGTGCAGAATAA	20	20	50.0	50.0	57.9	58.0	0.0	0.6	0.093532	56447	58456	2938-2976	0.625000	73.5
NQW19_pgp044_p2	NQW19_pgp044	2	GCGTCACACATACACCCTAG	CGAACCGAGGTGCAGAATAA	20	20	55.0	50.0	58.1	58.0	0.0	0.6	0.082012	56447	58456	2817-2854	0.500000	66.1
NQW19_pgp044_p3	NQW19_pgp044	3	TTCTCTGCAGCAGTAGGAGT	CGAACCGAGGTGCAGAATAA	20	20	50.0	50.0	58.1	58.0	22.1	0.6	0.074332	56447	58456	2494-2502	0.250000	52.8
NQW19_pgp044_p4	NQW19_pgp044	4	GGGGGATATTTCTCTGCAGC	CGAACCGAGGTGCAGAATAA	20	20	55.0	50.0	58.1	58.0	9.5	0.6	0.101645	56447	58456	2503-2511	0.250000	52.6
NQW19_pgp044_p5	NQW19_pgp044	5	TTCTCTGCAGCAGTAGGAGT	ACCTCCCCAAGACTTCGTTA	20	20	50.0	50.0	58.1	58.0	22.1	0.0	0.089557	56447	58456	2793-2801	0.250000	52.4
NQW19_pgp044-NQW19_pgp043_p1	NQW19_pgp044-NQW19_pgp043	1	CTCGTTTTCTACTTCGGGGG	ACCTCCCCAAGACTTCGTTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025778	58457	58972	1226-1242	0.625000	72.8
NQW19_pgp044-NQW19_pgp043_p2	NQW19_pgp044-NQW19_pgp043	2	TTAATTGCGACGAGACAGGG	ACCTCCCCAAGACTTCGTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024390	58457	58972	914-919	0.500000	63.7
NQW19_pgp044-NQW19_pgp043_p3	NQW19_pgp044-NQW19_pgp043	3	ACTAAACGCCCCAACACATT	ACCTCCCCAAGACTTCGTTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.030101	58457	58972	855-855	0.500000	63.4
NQW19_pgp044-NQW19_pgp043_p4	NQW19_pgp044-NQW19_pgp043	4	CTCGTTTTCTACTTCGGGGG	GTTCCCGGTGATACTCCAAC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.025918	58457	58972	1256-1260	0.375000	58.8
NQW19_pgp044-NQW19_pgp043_p5	NQW19_pgp044-NQW19_pgp043	5	TTAATTGCGACGAGACAGGG	GTTCCCGGTGATACTCCAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.024530	58457	58972	932-937	0.375000	58.2
NQW19_pgp039_p1	NQW19_pgp039	1	CTTCATGCTCGTCGAAAGGA	TTCGTTGAATTGGGGCTTCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.015415	61563	63023	2751-2763	0.375000	63.8
NQW19_pgp039_p2	NQW19_pgp039	2	TCTTCATGCTCGTCGAAAGG	TTCGTTGAATTGGGGCTTCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.015415	61563	63023	2752-2764	0.375000	63.8
NQW19_pgp039_p3	NQW19_pgp039	3	TTTGATCTGGGAGGCAGAGA	TTCGTTGAATTGGGGCTTCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.054838	61563	63023	2827-2833	0.375000	59.0
NQW19_pgp039_p4	NQW19_pgp039	4	CTAAGTCCCGTTGAAGGTCG	TTCGTTGAATTGGGGCTTCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.019537	61563	63023	2740-2742	0.250000	52.7
NQW19_pgp039_p5	NQW19_pgp039	5	CTTCATGCTCGTCGAAAGGA	GAGTGAGGAGAGGGAGAGAC	20	20	50.0	60.0	58.0	57.9	0.0	0.0	0.056491	61563	63023	2708-2708	0.125000	44.5
NQW19_pgp038_p1	NQW19_pgp038	1	TCGCAAACAAGGTATCTCGG	ACGGTACAGACGACGATTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031444	63233	64401	1879-1899	0.625000	71.6
NQW19_pgp038_p2	NQW19_pgp038	2	TCGCAAACAAGGTATCTCGG	CGATCGAGTATCAGCAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	10.3	0.042171	63233	64401	1698-1718	0.625000	71.4
NQW19_pgp038_p3	NQW19_pgp038	3	TCGCAAACAAGGTATCTCGG	CTCGATCGAGTATCAGCAGC	20	20	50.0	55.0	58.0	58.0	0.0	32.2	0.042171	63233	64401	1700-1720	0.625000	71.4
NQW19_pgp038_p4	NQW19_pgp038	4	TCGCAAACAAGGTATCTCGG	AACGGATCTATCTGGGGGAG	20	20	50.0	55.0	58.0	58.0	0.0	3.9	0.021487	63233	64401	1486-1506	0.375000	64.7
NQW19_pgp038_p5	NQW19_pgp038	5	TCGCAAACAAGGTATCTCGG	GAACGGATCTATCTGGGGGA	20	20	50.0	55.0	58.0	58.0	0.0	3.9	0.015705	63233	64401	1507-1507	0.125000	44.9
NQW19_pgp031_p1	NQW19_pgp031	1	CAATCCATAAGGGGCAGCAT	AACCGGTAATGAGGGGGTTA	20	20	50.0	50.0	58.0	58.0	0.0	8.0	0.035658	68306	69612	1724-1724	0.125000	44.7
NQW19_pgp031_p2	NQW19_pgp031	2	GGTCAATCCATAAGGGGCAG	AACCGGTAATGAGGGGGTTA	20	20	55.0	50.0	58.0	58.0	0.0	8.0	0.043405	68306	69612	1727-1727	0.125000	44.7
NQW19_pgp031_p3	NQW19_pgp031	3	CAATCCATAAGGGGCAGCAT	GAATGGGCCAGAGAAGGAAG	20	20	50.0	55.0	58.0	58.0	0.0	13.8	0.055259	68306	69612	1657-1657	0.125000	44.6
NQW19_pgp031_p4	NQW19_pgp031	4	CAATCCATAAGGGGCAGCAT	GGGACGCCTTAATGGTAGTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.055905	68306	69612	1691-1691	0.125000	44.6
NQW19_pgp031_p5	NQW19_pgp031	5	AGGGGCAGCATATCTTTTCG	AACCGGTAATGAGGGGGTTA	20	20	50.0	50.0	58.0	58.0	0.0	8.0	0.057415	68306	69612	1715-1715	0.125000	44.5
NQW19_pgp027_p1	NQW19_pgp027	1	GGAGTAACACGCCCTGTAAG	AAACTGGGAACGGGAGAAAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.028914	71279	72852	2406-2406	0.500000	65.7
NQW19_pgp027_p2	NQW19_pgp027	2	GGAGTAACACGCCCTGTAAG	TTTCGTGTATGTCGCAGGAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.015171	71279	72852	2183-2183	0.250000	52.7
NQW19_pgp027_p3	NQW19_pgp027	3	CCCGTCCATTGTGAATAGCA	TTTCGTGTATGTCGCAGGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046158	71279	72852	1891-1891	0.250000	52.7
NQW19_pgp027_p4	NQW19_pgp027	4	GGAGTAACACGCCCTGTAAG	AAGAGGGAACTACACGGGAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025778	71279	72852	2485-2485	0.125000	44.8
NQW19_pgp027_p5	NQW19_pgp027	5	GGAGTAACACGCCCTGTAAG	AGAGGGAACTACACGGGAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025778	71279	72852	2484-2484	0.125000	44.8
NQW19_pgt020_p1	NQW19_pgt020	1	AACCAACCACCCATTCTGTC	TGGAACCTTGAACAGACTGC	20	20	50.0	50.0	58.0	58.0	0.0	1.2	0.037305	83272	84309	1943-1976	0.875000	86.7
NQW19_pgt020_p2	NQW19_pgt020	2	AACCAACCACCCATTCTGTC	GACTCATTTGGCATCTCGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034462	83272	84309	1527-1552	0.750000	80.5
NQW19_pgt020_p3	NQW19_pgt020	3	AACCAACCACCCATTCTGTC	TCTTTCCTTGGTGAAAGCCC	20	20	50.0	50.0	58.0	58.0	0.0	8.3	0.009247	83272	84309	1372-1397	0.625000	71.3
NQW19_pgt020_p4	NQW19_pgt020	4	CGGTTTTTCCCCTCCTTCAA	TCTTTCCTTGGTGAAAGCCC	20	20	50.0	50.0	58.0	58.0	0.0	8.3	0.014917	83272	84309	1743-1761	0.500000	64.5
NQW19_pgt020_p5	NQW19_pgt020	5	CGGTTTTTCCCCTCCTTCAA	GACTCATTTGGCATCTCGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040132	83272	84309	1898-1916	0.500000	64.3
NQW19_pgp020-NQW19_pgt022_p1	NQW19_pgp020-NQW19_pgt022	1	AAAGGGATAAGGACGGGACA	TCCACATCGATTCGTGAACC	20	20	50.0	50.0	58.0	58.0	0.0	15.4	0.043450	100976	101714	1682-1734	0.750000	81.7
NQW19_pgp020-NQW19_pgt022_p2	NQW19_pgp020-NQW19_pgt022	2	AAGGGATAAGGACGGGACAA	TCCACATCGATTCGTGAACC	20	20	50.0	50.0	58.0	58.0	0.0	15.4	0.043450	100976	101714	1681-1733	0.750000	81.7
NQW19_pgp020-NQW19_pgt022_p3	NQW19_pgp020-NQW19_pgt022	3	AGGGATAAGGACGGGACAAA	TCCACATCGATTCGTGAACC	20	20	50.0	50.0	58.0	58.0	0.0	15.4	0.043450	100976	101714	1680-1732	0.750000	81.7
NQW19_pgp020-NQW19_pgt022_p4	NQW19_pgp020-NQW19_pgt022	4	AAGGGATAAGGACGGGACAA	TTGAGTCACCAACCCCATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044911	100976	101714	1967-1987	0.625000	69.4
NQW19_pgp020-NQW19_pgt022_p5	NQW19_pgp020-NQW19_pgt022	5	AGGGATAAGGACGGGACAAA	TTGAGTCACCAACCCCATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044911	100976	101714	1966-1986	0.625000	69.4
NQW19_pgp005-NQW19_pgr004_p1	NQW19_pgp005-NQW19_pgr004	1	CGTTGGTTTATAGGGCGACA	TCTATCACCACTGGTTTGCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.106243	149382	149501	675-675	0.125000	44.2
NQW19_pgp005-NQW19_pgr004_p2	NQW19_pgp005-NQW19_pgr004	2	ACGTTGGTTTATAGGGCGAC	TCTATCACCACTGGTTTGCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.107642	149382	149501	676-676	0.125000	44.1
NQW19_pgp005-NQW19_pgr004_p3	NQW19_pgp005-NQW19_pgr004	3	GAACAAACACGCCAACTAGC	TCTATCACCACTGGTTTGCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.125720	149382	149501	636-636	0.125000	44.0
NQW19_pgp005-NQW19_pgr004_p4	NQW19_pgp005-NQW19_pgr004	4	GTACCTGGTCCCTTTCGAAC	TCTATCACCACTGGTTTGCG	20	20	55.0	50.0	57.9	57.9	0.6	0.0	0.183706	149382	149501	735-735	0.125000	43.5
NQW19_pgp005-NQW19_pgr004_p5	NQW19_pgp005-NQW19_pgr004	5	CGTTGGTTTATAGGGCGACA	CTATCACCACTGGTTTGCGT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.204526	149382	149501	674-674	0.125000	43.4