Hotspot

rpl16

LSC · 77347-78839 · 1493 bp

Back to Schizachyrium

Pi 0.0109

MarkerSeek score 58.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1493
variable_sites: 86
pi: 0.0109
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	GGGTCGTCTAGCAGGAAAAG	55.0	55.0	57.9	58.0	1953-2023 bp	1.0	87.5
rpl16_p2	TTTCTAGAGGCATTTGGGGC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	2025-2095 bp	1.0	86.7
rpl16_p3	TCAGCAATAGTGTCCTTGCC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	2396-2466 bp	1.0	86.0
rpl16_p4	TTATACCGTCGTCGCCTTTG	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	58.1	58.0	2091-2161 bp	0.975	86.0
rpl16_p5	GCAATAGTGTCCTTGCCCAT	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	58.2	58.0	2393-2463 bp	1.0	85.1