Hotspot

rpl32-trnL-UAG

SSC · 106069-106599 · 531 bp

Back to Schizachyrium

Pi 0.0142
MarkerSeek score 63.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
531
variable_sites
52
pi
0.0142
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTTTCTGGGCAACAAGC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.4 58.0 571-657 bp 1.0 87.3
rpl32-trnL-UAG_p2 GGTTTTTCTGGGCAACAAGC GGATTCGAACCGAGATGCTT 50.0 50.0 58.4 58.1 574-660 bp 1.0 86.9
rpl32-trnL-UAG_p3 GGTTTTTCTGGGCAACAAGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.4 57.9 544-630 bp 1.0 86.5
rpl32-trnL-UAG_p4 GGTTTTTCTGGGCAACAAGC AGCAGCGTGTCTACCAATTT 50.0 45.0 58.4 57.8 537-623 bp 1.0 86.1
rpl32-trnL-UAG_p5 GGTTTTTCTGGGCAACAAGC GCTTGAGCACTGCTTCCTAA 50.0 50.0 58.4 58.2 558-644 bp 1.0 85.9